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Zymo Research
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Thermo Fisher
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Immagina Biotechnology SRL
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Thermo Fisher
70 u of fastap and processed analogously as the untreated samples until proteins from the polysome profile fractions were precipitated 70 U Of Fastap And Processed Analogously As The Untreated Samples Until Proteins From The Polysome Profile Fractions Were Precipitated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/70 u of fastap and processed analogously as the untreated samples until proteins from the polysome profile fractions were precipitated/product/Thermo Fisher Average 90 stars, based on 1 article reviews
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Illumina Inc
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Santa Cruz Biotechnology
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Mendeley Ltd
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Teledyne LABS
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Brandel Inc
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MedChemExpress
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Journal: Cell reports
Article Title: Kaposi’s sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis
doi: 10.1016/j.celrep.2021.110144
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Immunohistochemistry, Virus, Recombinant, Lysis, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Amplification, Reverse Transcription, Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Control, Software, Real-time Polymerase Chain Reaction, Western Blot
Journal: Cancer Research
Article Title: LncRNA HIF1A-AS1 Promotes Gemcitabine Resistance of Pancreatic Cancer by Enhancing Glycolysis through Modulating the AKT/YB1/HIF1α Pathway
doi: 10.1158/0008-5472.can-21-0281
Figure Lengend Snippet: Figure 4. HIF1A-AS1 promotes HIF1a translation via recruiting RNA binding protein YB1. A, Combined immunofluorescence of the nucleolar marker protein nucleolin (yellow) and RNA-FISH analysis of HIF1A-AS1 (green) and HIF1a mRNA (red) in BxPC3/PANC1GEM-R cells. B, Schematic of affinity purification and detection of MS2-tagged HIF1A-AS1. C, GEM-R cells were transfected with the MS2 fusion plasmid system and then immunoprecipitation was performed using anti-GST antibody. The relative enrichment of HIF1a mRNA in the beads of MS2 group or HIF1A-AS1-MS2 group was assessed by qRT-PCR. D, RNA interaction profile from catRAPID suggesting that HIF1A-AS1 binds to the YB1 protein. E and F, Total cellular proteins of GEM-R cells were used in a biotin-labeled RNA pull-down assay, and YB1 binding to HIF1A-AS1 (E) or HIF1a mRNA (F) was detected via Western blot. Bio-AS1/HIF1a mRNA, transcribed and labeled HIF1A-AS1/HIF1a mRNA; No bio-AS1/HIF1a mRNA, transcribed but not labeled HIF1A-AS1/HIF1a mRNA; Ctrl RNA, transcribed and labeled antisense of HIF1A-AS1/HIF1a mRNA. G, BxPC3GEM-R cell lysates were mixed with biotinylated HIF1A-AS1 RNA or antisense (dotted line) fragments, then RNA pull-down assay was performed. (Continued on the following page.)
Article Snippet:
Techniques: RNA Binding Assay, Marker, Transfection, Plasmid Preparation, Immunoprecipitation, Quantitative RT-PCR, Labeling, Pull Down Assay, Binding Assay, Western Blot